Notes on Tissue Culture.

ButterflyDreams · 11-07-2000, 02:57 PM

Hola Gang.

Found this at http://www.onthenet.com.au/~rfossard...gan%20Cultures

Dr. RONALD A. de FOSSARD, from Australia has been experimenting with tissue culture of plants for 30+ years. Some of the things in the article lead to interesting ideas when considered with Cannabis in the equation.

I thought the part below might be of interest.

BD

Quote:

Anther and Pollen Grain Culture
The culture of immature anthers and pollen grains is done so as to induce the pollen grains to develop into multicellular forms, particularly into embryos, with half the normal number of chromosomes for the species. When such haploid embryos are treated with chromosome doubling agents, e.g. colchicine, their normal chromosome number is restored (and thus their fertility) and, in addition, the plants are pure lines. Pure line formation is a natural tendency for self fertilizing species and can be obtained with cross fertilizing species with repeated in-breeding for 10 or more generations.

So far the induction of haploid plant formation from anther and pollen grain cultures has been successful mainly with naturally self fertilizing species and thus, on chromosome doubling, are in theory very similar if not identical with the parents. However, by first crossing many lines from a self fertilizing species, new combinations of genes are formed, and haploid plants produced by anther culture from such crosses could be an extremely valuable and quick way of obtaining pure lines of these new combinations.

If we can find out how to obtain haploid plants from anther culture of cross fertilizing species, they also could be extremely valuable relative to breeding programmes and the selection of improved strains.

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