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Old 03-02-2007, 10:30 AM   #1
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tweaking cloning method
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I have been very satisfied with cloning in glass of water. I recently tried three different solutions.

I cut each clone at an extreme angle to expose as much of the marrow (so to speak) inside the stem. I use 6" tinted glass tumblers (16oz), 3 are tinted green, one tinted pink. They seem to do better than clear glass. The length of the clone is just over 6" so the upper most fan leave hangs just over the rim. This seems to hold the clone upright.

The three solutions are as follows:

1. straight bottled water - pH 7.2, room temperature
2. same water (with the amount of rooting hormone that would
cling to the bottom inch of a wetten clone), dissolved and
strained to fill bottom 1 1/2" of glass.
3. a 50/50 solution made up of same water and bloom solution
to cover bottom 1 1/2" of glass.

Each glass has two clones in it, one has the bottom 1 1/2 inches shaved, while the over is just cut and placed into the glass.

So far, the plain water, unshaven clone was first to root, all others are showing nubs.
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Old 03-02-2007, 10:55 AM   #2
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Mary Jane
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Are you leaving the clones in 24hr light or how are you doing it.

I have never cloned MJ and had thought to clone the tops of some shown females, but didnŽt.

Now I have another female IŽd like to clone maybe.

Have you experienced cloning a female when just showing sex and further in?

Just interested in your experiments and findings!

Peace

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Old 03-02-2007, 11:08 AM   #3
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Once they're in the tinted glass, I place them in the same light cycle they came from (18/6). I keep them away from direct light, usually under a shelf. I also like to shield the lower two inches of the glass from any kind light.

Last edited by OCT_420; 03-02-2007 at 11:23 AM..
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Old 03-03-2007, 02:01 PM   #4
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Results with photos
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I hope this helps some of you who are afraid to try cloning. I'm in no hurry for results, the air temp in the grow room goes from 74 when the lights are on, to 60 when off. Light cycle is 18/6. These pictures are taken after 14 days. I know there are methods out that give faster results but with very little effort on my part, I can obtain 100% cloning rate.
It appears I will be using the methods used with B,C,E from now on.

Six Clones - three tinted vessels - three solutions.

2 clones in each glass - one shaven, one not

3 solutions - plain bottled H2O pH 7.2, Bloom solution, hormone solution

6 clones - 3 w/ bottom 1 1/2" shaven, 3 not (1 shaven, 1 not, in each glass)

1st pic shows the six clones in their containers in indirect light, with their roots sheilded by the gallon jugs.

2nd pic - F-water unshaven - was the first to show nubs then roots.

3rd pic - G-water shaven - notice only two little nubs

4th pic - D-bloom unshaven - roots only sprouting from very bottom

5th pic - E-bloom shaven - nice collection of roots

6th pic - B-hormone unshaven- very nice

7th pic - C-hormone shaven - again very nice
Attached Thumbnails
tweaking-cloning-method-indirect-light-roots-shielded.jpg   tweaking-cloning-method-f-water-unshaven.jpg   tweaking-cloning-method-g-water-shaven.jpg  

tweaking-cloning-method-d-bloom-unshaven.jpg   tweaking-cloning-method-e-bloom-shaven.jpg   tweaking-cloning-method-b-hormone-unshaven.jpg  

tweaking-cloning-method-c-hormone-shaven.jpg  
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Old 03-03-2007, 02:46 PM   #5
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Hi OCT_420

Good job!

For the "shaven" cuttings, it looks like all or almost all of the roots emerged from the base of the cuttnig, and from the top of the "shaved" area (from the bottom edge of the unshaved epidermis).

Roots originate from undifferntiated tissue, which is normallly found in the meristem (axillary meristem at the nodes); the layer of cells immediately adjacent to the callus that forms over a cut is also udifferentiated. So I think you will get very similar results by not removing any epidermis, but making sure that there's a node or two in the solution. Roots should emerge from the node faster, because there's no delay waiting for the callus to form.


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Old 03-03-2007, 03:21 PM   #6
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Very informative cloning post- pics are great too. One question for you, did you use a humidity dome and just take it off for the pics, or is one unecessary? I've had most success cloning in dirt, but now I really want to try this out.
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Old 03-03-2007, 05:55 PM   #7
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No dome needed
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Originally Posted by Magical Cabinet View Post
Very informative cloning post- pics are great too. One question for you, did you use a humidity dome and just take it off for the pics, or is one unecessary? I've had most success cloning in dirt, but now I really want to try this out.
I've spent alot on time on this board reading, alot of different methods, I read a great majority of them. I tried to take a little from several different methods. I tried to find a method that would require as little as possible from me.

I cut, recut, place in 2" of water (I think now I will be using the disolved hormone or the bloom solution), place somewhere out of direct light, shield bottom 2" from any light. In the summer I set them on a north facing window sill. (the reverse in the sothern hemisphere).

I check them daily, add water to the 2" level. I usually wait til the roots are about 2" long before I put them in soil. Hope this helps.
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Old 03-03-2007, 06:01 PM   #8
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what you speak of as 'marrow' may in fact be 'phloem'.
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Old 03-04-2007, 02:04 PM   #9
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what you speak of as 'marrow' may in fact be 'phloem'.
Plant biology here I come.
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Old 03-05-2007, 05:55 PM   #10
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And here we go!
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Well, I figured I may as well post the photos of the girls planted in 5" pots. One of the seven is very slow to root, just that little nub, I hate to give her the old heeve ho.

I've upgraded my lights to 3 - 85watt (350w equivilant) 6500K CFL (veggies). I'm hoping to see a better yield than the 40w tubes. There are another 3x85w 3000K CFL in the flower cubby hole.

Guess I can only wait and see.
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