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Go Back   The Garden's Cure > Advanced Horticulture > Theories & Speculation
Reload this Page Creating the Superclone-Colchicine Experiment
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Old 01-21-2008, 03:56 PM   #41
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First 3 pics are the Sativa group.
Had to pry the seed jacket off of the little runt in the second pic.

Pic 4 is the control group. Just a few of the treated sets are close in ht.

Pics 5-7 are some of the interesting Indicas/runts.

Last pic is a group shot of the Indicas with the control in the upper right.
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Old 01-23-2008, 12:30 AM   #42
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The seed would have to absorb some of the chemical.

But really how much liquid can a seed absorb?

1mL? Less? More?

I'm sure its not enough to harm you.
I would be willing to agree also that once the chemical
is in the plant, it will not be able to reproduce the chemical in any way.

So where will the chemical go? Tiny amounts in every cell? Concentrated in the stalk? Buds? Leaves?

I just don't think there could be enough there to hurt you

But then again... I'm no professional
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Old 01-23-2008, 09:27 AM   #43
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Old 01-23-2008, 09:45 AM   #44
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Quote:
Originally Posted by green thumb View Post
You are DEAD wrong, if you do not know something just shut up.
I have an account with VWR, and colchine itself is toxic as hell.
But I an not a biologist so I'm seeking some info . . .

google is your friend
Let's keep the thread civil. We are all just asking questions here.
I am playing on the safe side of the street, in any case.
Colchicine is toxic to a degree. But people injest .6 mg at a shot.

What we don't know is what happens in the plant once it is treated with the drug.
It may be that some funky interaction happens that creates a compound that is harmfull when smoked. (I doubt it)
Maybe colchicine is safe when injested but not when smoked.

We do not have answers to these questions.
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Old 01-23-2008, 11:22 AM   #45
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Sounds like we need a chemist or a biologist.
Actually a pharmacist would probably work well.

What types of mutations are you looking for exactly? More budsites?
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Old 01-23-2008, 12:57 PM   #46
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no, no; my post was in error, I pulled it and appologized to matrin

I need to google to find the concentration used in "scientific" studies, perhaps it is far higher than that using gout pills ?

Is there a specific carcinogen (or such) as a product of combustion ?

Addendum
The (potential) effects of exposing a plant to colchine are well described, I have never seen a quantitive description of uptake or persistance or breakdown; is colchine even stable within the plant ?

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Old 01-23-2008, 02:59 PM   #47
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This seems to be how the big boys do it, an excerpt:
(from google search)
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Old 01-23-2008, 03:23 PM   #48
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The top paragraph of the second thumbnail above would seem to provide the answer.
"It falls under Ferguson's second category of compounds and the reaction is chemical."

To me this means that the colchicine penetrates the cell wall and has a chemical reaction after which it is no longer colchicine, merely the resultant materials of the reaction.

I would be careful of treated seeds, but I cannot conceive of colchicine in the resultant plant.

We need an opinion from a Biologist.
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Old 01-23-2008, 03:25 PM   #49
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From what I see of the excerpts above is perhaps another use for colchicine.
It appears they are applying solutions to plant and animal cells to observe results on said cells in a lab. It doesn't seem that they are growing cells into an organizisim.

I think what we are talking about is a bit different.
Early on in this thread are links to volumes on the treatment of marijuana with colchicine.
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Old 01-23-2008, 03:59 PM   #50
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I think their use was quite the same, to promote cell division.
They treated seeds or used a folar spray (not recommended at all as we want the whole plant the same, but different, way).
They then looked at the cells, which seems to be the only in which polyploidism can be definitively determined.

If a material is a part of a chemical reaction, it does not remain unchanged; ipso facto there can be no colchicine in the plant matter grown from a treated seed (from whence did it come ?).
But all may interpret the excerpt as they will.
And better to err on the sire of prudence in any case.

xyz
Did you note the dosage of 0.01 - 1.0% (though Table 1 indicates 0.5 - 1.0%) ?
Your soln works out to 0.07%, FWIW
As I understand it; the concentration, effect, and mortality are all directly related.
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